Journal: Journal of autoimmunity

Article Title: Innate immunity in myasthenia gravis thymus: pathogenic effects of Toll-like receptor 4 signaling on autoimmunity.

doi: 10.1016/j.jaut.2013.12.013

Figure Lengend Snippet: Fig. 10. Proposed model for TLR4-mediated intra-thymic pathogenesis of MG. In the context of genetically susceptible backgrounds, a “danger signal” in the thymus, such as pathogen infection, may trigger the activation of TLR4-mediated signaling pathways, which in turn may lead to pathogenic and self-perpetuating inflammatory autoimmune response. Specifically, persistent activation of TLR4 in TECs and thymic DCs may promote an inflammatory state that contributes to increased expression, presentation and cross- presentation of AChR, leading to T-cell autosensitization and development of the autoimmune response. Close interactions between T-cells and APCs at the medullary HC areas could be critical for initiation of AChR-specific autoimmunity. Increased production of pro-inflammatory chemokines (e.g. CXCL13, CCL21, CCL17 and CCL22) may cause abnormal recruitment of DCs, T- and B-cells in the thymus, leading to hyperplastic changes and perpetuating the inflammatory state. Th17-related cytokines produced in the inflammatory microenvironment may impair Treg function, thus favoring perpetuation of the inflammatory process and contributing to the feedback loop that promotes maintenance of the autoimmune response in MG thymus.

Article Snippet: FifteenTh17-relatedproteins were measured by the Bio-Plex Pro Human Th17 Cytokine Assay kit (Bio-Rad Laboratories), following manufacturer’s instructions.

Techniques: Infection, Activation Assay, Protein-Protein interactions, Expressing, Produced

Journal: Cancer Management and Research

Article Title: High Level of IL-10 in Cerebrospinal Fluid is Specific for Diagnosis of Primary Central Nervous System Lymphoma

doi: 10.2147/CMAR.S255482

Figure Lengend Snippet: Clinical Characteristics and Th17 Cell-Related Cytokine Levels in the CSF of 108 Patients

Article Snippet: The CSF levels of Th17 cell-related cytokines (including IL-1β, IL-4, IL-6, IL-10, IL-17A, IL-17F, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, IFN-γ, sCD40L, TNF-α and so on) were measured in 108 patients by Bio-Plex Pro TM Th17 Cytokine Assay produced by Bio-Rad Laboratories, Inc.

Techniques:

Journal: Cancer Management and Research

Article Title: High Level of IL-10 in Cerebrospinal Fluid is Specific for Diagnosis of Primary Central Nervous System Lymphoma

doi: 10.2147/CMAR.S255482

Figure Lengend Snippet: The AUC of Th17 Cell-Related Cytokine CSF Levels in 108 Patients

Article Snippet: The CSF levels of Th17 cell-related cytokines (including IL-1β, IL-4, IL-6, IL-10, IL-17A, IL-17F, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, IFN-γ, sCD40L, TNF-α and so on) were measured in 108 patients by Bio-Plex Pro TM Th17 Cytokine Assay produced by Bio-Rad Laboratories, Inc.

Techniques:

Journal: BMC Immunology

Article Title: Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells

doi: 10.1186/s12865-016-0182-8

Figure Lengend Snippet: Intra-assay precision of samples. %CV of the concentrations (mean CVs for four donors calculated using triplicate cultures for each cytokine) as determined with Luminex magnetic kits from Ozyme, Millipore, and Bio-Rad for the 11 common analyzed cytokines. Left panel: Non-stimulated PBMC; middle panel: PPD-stimulated PBMC; right panel: SEB-stimulated PBMC. Mean CVs calculated for all donors and all cytokines are represented by red lines

Article Snippet: We compared the following commercial kits: one 16-plex magnetic Procarta Immunoassays kit (Affymetrix) from Ozyme; one 15-plex Milliplex MAP human cytokine magnetic bead panel kit (#SPR158) and one simplex Milliplex MAP human cytokine/chemokine magnetic bead panel, both from Millipore; one Bio-Plex Pro assays magnetic kit and one Bio-Plex Pro human Th17 cytokine assays kit, both from Bio-Rad.

Techniques: Intra Assay, Luminex

Journal: BMC Immunology

Article Title: Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells

doi: 10.1186/s12865-016-0182-8

Figure Lengend Snippet: Cytokine profiles in TB-infected patient. Cytokine production by PPD- and ESAT-6-stimulated PBMC from a TB-infected donor was measured with kits from Millipore, Ozyme and Bio-Rad. a Specific cytokine Concentrations (background values subtracted). b Stimulation Index (ratio between stimulated and background concentration values). The value for IL-22 from non-stimulated PBMC given by the Millipore kit was inferior to the range of the standard curve and was therefore imputed by the lowest extrapolated value for this cytokine (3.1 pg/ml). The IL-17A concentration with ESAT-6 stimulation was a null value for all kits, so SI could not be calculated. The positive threshold for the SI was set at 3 (black line)

Article Snippet: We compared the following commercial kits: one 16-plex magnetic Procarta Immunoassays kit (Affymetrix) from Ozyme; one 15-plex Milliplex MAP human cytokine magnetic bead panel kit (#SPR158) and one simplex Milliplex MAP human cytokine/chemokine magnetic bead panel, both from Millipore; one Bio-Plex Pro assays magnetic kit and one Bio-Plex Pro human Th17 cytokine assays kit, both from Bio-Rad.

Techniques: Infection, Concentration Assay

Journal: BMC Immunology

Article Title: Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells

doi: 10.1186/s12865-016-0182-8

Figure Lengend Snippet: Description of Luminex kits

Article Snippet: We compared the following commercial kits: one 16-plex magnetic Procarta Immunoassays kit (Affymetrix) from Ozyme; one 15-plex Milliplex MAP human cytokine magnetic bead panel kit (#SPR158) and one simplex Milliplex MAP human cytokine/chemokine magnetic bead panel, both from Millipore; one Bio-Plex Pro assays magnetic kit and one Bio-Plex Pro human Th17 cytokine assays kit, both from Bio-Rad.

Techniques: Luminex